Human pluripotent stem cells as models for cardiovascular disease

Datum

17.02.2017

Zeit

11:00 - 12:00

Sprecher

Prof. Dr. Christine Mummery

Zugehörigkeit

Leiden University,Medical Center

Serie

CMCB Life Sciences Seminar

Sprache

en

Hauptthema

Biologie

Andere Themen

Biologie, Medizin

Host

Dr. Cristina Golfieri

Beschreibung

About the research of Prof. Mummery:Derivation of many different cell types from human pluripotent stem cells is an area of growing interest both for potential cell therapy and as a platform for drug discovery and toxicity. The recent availability of methods to introduce specific disease mutations into human pluripotent stem cells and/or to derive these cells as hiPS cells by reprogramming from any patient of choice, are creating unprecedented opportunities to create disease models “in a dish” and study ways to treat it or slow down its rate of development. We have used these methods to produce isogenic pairs of hiPSC lines to compare diseased and corresponding control cardiomyocytes and vascular endothelial cells and identify disease related phenotypes and mechanisms. We have examined drug responses of hESC-derived cardiomyocytes to a variety of cardiac and non-cardiac drugs and shown that iPSC derived cardiomyocytes with mutations in ion channel genes can accurately predict changes in cardiac electrical properties and reveal drug sensitivities also observed in patients. The implications of these studies will be discussed.5 most important publications:1. Lebrin F, Srun S, Raymond K, Martin S, van den Brink S, Freitas C, Bréant C, Mathivet T, Larrivée B, Thomas J-L, Arthur HM, Westermann CJJ, Disch F, Mager JJ, Snijder RJ, Eichmann A, Mummery CL. Thalidomide stimulates vessel maturation and reduces epistaxis in Hereditary Hemorrhagic Telangiectasia patients. Nature Medicine 2010 16(4):420-8.2. Bellin M, Marchiotti C, Gage F, Mummery CL. Induced pluripotent stem cells: the new patient? Nature Rev Mol Cell Biol., 2012. 13(11):713-263. Bellin M, Davis RP, Casini S, D’Aniello C, Haas J, Ward-van Oostwaard D, Tertoolen LGJ, Jung CB, Elliott DA, Welling A, Laugwitz KL, Moretti A, Mummery CL Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome. EMBO J 2013 32, 3161–3175.4. V.V. Orlova, F.E. van den Hil, S. Petrus-Reurer, Y. Drabsch, P. ten Dijke, C.L. Mummery. Endothelial Cells and Pericytes from human Pluripotent Stem Cells: methods for efficient generation, expansion and examination of functional competence. Nature Protocols 2014 9, 1514-15315. Birket MJ, Ribeiro MC, Verkerk AO, Ward D, Leitoguinho AR, den Hartogh SC, Orlova VV, Devalla HD, Schwach V, Bellin M, Passier R & Mummery CL. Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells. Nature Biotechnol. 2015 33(9):970-9.

Links

• to announcement of the organizer

Letztmalig verändert: 17.02.2017, 08:49:25