Speaker: Louis-Philippe Bergeron-Sandoval University of Montreal, Canada Title: "Cells Can Temporally Resolves Specific versus Promiscuous Phosphorylation"

Datum

28.06.2017

Zeit

11:00 - 12:00

Sprecher

Louis-Philippe Bergeron-Sandoval

Zugehörigkeit

University of Montreal, Canada

Sprache

en

Hauptthema

Biologie

Andere Themen

Biologie

Host

Anthony Hyman

Beschreibung

How can we understand cell signaling networks that seem irreducibly complex? Protein kinases, the major signaling enzymes in the cell, form a highly connected and complex network. We have argued that complexity of the kinase network may have evolved to integrate and compare multiple signals, resulting in main and complementary responses to stimuli. Such a network could also generate noise, or promiscuous phosphorylation that has no functional consequences but confound understanding of the signaling response. We hypothesize that phosphorylation of specific substrates occurs rapidly following stimulation but that promiscuous phosphorylation take place more slowly, following random encounters with neighboring proteins. Therefore, capturing early signaling events (phosphorylation or dephosphorylation) immediately after cell stimuli could facilitate the detection of stimulus-specific phosphorylation events. To test this hypothesis, we performed high temporal and coverage (5-s resolution) phosphoproteomics analyses to yield unprecedented profiling of high-osmolarity glycerol (HOG) response in budding yeast. We show that conserved and putatively functional kinase- or phosphatase-substrate interactions in the HOG response do occur more rapidly than promiscuous interactions. We also provide a rich dataset of dynamic phosphosites that may be implicated in the regulation of the cell cycle, cytoskeletal dynamics and morphogenesis

Letztmalig verändert: 29.06.2017, 10:00:05