In toto imaging and dynamic reconstruction of post-implantation mouse development at the cellular level using light-sheet microscopy

Datum

02.04.2019

Zeit

11:00 - 12:00

Sprecher

Kate McDole

Zugehörigkeit

HHMI, Janelia Research Campus, Ashburn, VA, USA

Sprache

en

Hauptthema

Biologie

Andere Themen

Biologie

Host

Marino Zerial

Beschreibung

The rapid advancement of light-sheet microscopy and computational methods for analyzing complex image data sets has provided an unprecedented opportunity to systematically study the development and morphogenesis of the mouse embryo at the single-cell level. We have developed a light-sheet system capable of continuously imaging, and adapting to, the entire mouse embryo during these stages with high spatiotemporal resolution for days at a time. We are able to follow the dynamic behaviors of both individual cells and populations among the different germ layers, and visualize the large-scale morphodynamic events that pattern different tissues. In combination with a suite of software tools we have developed, we are able to track individual cells and build dynamic cell fate maps, describe and analyze patterns of divisions during tissue formation, such as during neural tube elongation and closure, as well as examine the formation and migration of cells through the primitive streak, and the development and formation of a number of embryonic structures such as the node and notochord. These advances have enabled us to analyze the development of the post-implantation mouse embryo in unprecedented detail, offering new insights into this critical, yet poorly characterized area of mammalian development.

Letztmalig verändert: 03.04.2019, 02:09:56