Folding of nascent proteins on the ribosome

Datum

08.06.2023

Zeit

11:00 - 12:00

Sprecher

Marina Rodnina

Zugehörigkeit

Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany

Serie

MPI-CBG Thursday Seminar

Sprache

en

Hauptthema

Biologie

Host

Marino Zerial

Beschreibung

The ribosome is a macromolecular machine that synthesizes proteins and guides their folding towards the correct, functional structures. Defects in protein folding cause many human diseases; thus, understanding co-translational folding is of eminent importance. Cotranslational protein folding starting as soon as the N-terminal parts of nascent proteins become available, but is confined by the narrow space of the polypeptide exit tunnel of the ribosome. Compared to protein folding in solution, the ribosome alters the folding pathway, destabilizes native folds and stabilizes non-native intermediates of nascent proteins. Translation is a non-uniform process with periods of rapid translation interspersed with pauses. The stop-and-go rhythm of translation not only defines the rate with which a protein is synthesized, but also modulates folding and misfolding of nascent proteins. The sequence of the protein, the codon-specific translation rates and the interactions between the nascent peptide and the ribosome define the folding code of translation, which ultimately determine the quality of the cellular proteome.

Letztmalig verändert: 03.06.2023, 07:36:48