Apico-basal polarity disruption in patient-derived gastric organoids

Datum

17.05.2023

Zeit

16:00 - 17:00

Sprecher

Célia Galleri–Paris

Zugehörigkeit

Dye Lab - PoL

Sprache

en

Hauptthema

Biologie

Host

PoL Postdoc Series

Beschreibung

Apico-basal polarity is an important determinant of epithelium structure and orientation. Polarity is perturbed or lost in various epithelial cancers, often at the start of metastasis. Such polarity disturbance could be in part, due to the changes in the extracellular matrix (ECM) in the tumor microenvironment, which undergoes biomechanical modifications. Here we study the impact of the extracellular matrix on the apico-basal polarity of patient-derived gastric cancer organoids. We show that normal gastric organoids require interaction with the ECM in order to maintain normal polarity. Notably, they dramatically switch their polarity when the integrin β1 signaling pathway is inhibited. On the contrary, we find that cancer organoids inherently display heterogeneous phenotypes of polarity disturbance. We further discover that at least one gastric cancer line does not fully depend on the interaction with the ECM for maintaining polarity. We attempt to sub-clone this line and find potential evidence of genetic heterogeneity leading to such ECM independence

Letztmalig verändert: 18.05.2023, 07:38:47