From Single Molecules to Tissues: Application of Correlative BioAFM in Multi-User Microscopy Facilities

Datum

17.05.2023

Zeit

16:00 - 17:00

Sprecher

Dr. Torsten Müller

Zugehörigkeit

Bruker - PoL

Sprache

en

Hauptthema

Biologie

Host

PoL Postdoc Series

Beschreibung

Active forces in biological systems define the interactions between single molecules, growing cells and developing tissues. Over the last three decades atomic force microscopy (AFM) has become an indispensable tool for characterisation of biological systems, as it enables the structural and mechanical characterization of samples at near native/physiological conditions. Nevertheless, biological systems are typically very heterogeneous and dynamic, which could pose challenges during cell surface analysis or identification of molecules. We will discuss how correlative BioAFM is used in life science microscopy core facilities nowadays to understand the structural, functional, chemical, mechanical, and dynamic complexities of biological systems. We will introduce and give examples of how the combination of AFM imaging with advanced super-resolution optics leverages the advantages of immunolabelling techniques for truly correlative microscopy. Through an innovative sample stage design, we will show how a wide choice of scanners, together with optical tiling and multi-region AFM probing, enables multiparametric mechanical characterization of soft samples over a large area and provides additional optical data sets.

Letztmalig verändert: 18.05.2023, 07:38:48