Meteorins regulate the formation of the left- right organizer and the establishment of vertebrate body asymmetry

Datum

14.08.2023

Zeit

11:00 - 12:00

Sprecher

Fanny Eggeler

Zugehörigkeit

Institut de la Vision, Paris

Sprache

en

Hauptthema

Biologie

Host

Claudia Gerri

Beschreibung

During vertebrate embryonic development left-right symmetrybreaking is innated by a ciliated organ called the Node. Within the Node, a leftward flow of extraembryonic fluid named the Nodal flow mediates the asymmetric expressions of Nodal factors. Although downstream Nodal pathway components leading to the establishment of the embryonic leftright axis are well known, less is known about the development and formation of the embryonic Node itself. Here, we reveal a novel role for the Meteorin protein family in the establishment of the left-right axis and in the formation of the Kupffer’s vesicle, the Node equivalent structure in zebrafish. The CRISPR/Cas9 genetic inactivation of each or all three zebrafish Meteorin members family (metrn, metrnl1 and metrnl2) led to defects in the properties of the Kupffer’s vesicle (KV) caused by an impaired assembly and migration of the dorsal forerunner cells (DFCs) that shape the KV. As a consequence, we show that Metrns loss-of-function results in disturbed Nodal factors expression, notably leading to heart looping defects. We demonstrate that through the genetic interaction with the Integrins ItgV and Itg1b, Metrn proteins regulate the DFC clustering. Together, our results identify a new role for the Meteorin protein family in the left-right asymmetry patterning during early embryonic vertebrate development. With the goal of identifying Metrns putative receptor(s), we are currently investigating the cellular pathway(s) in which Meteorin proteins are involved in and are developing approaches to validate the identified candidates as bona fide Metrns receptor(s).

Letztmalig verändert: 15.08.2023, 07:40:03