Isogenic human-induced Pluripotent Stem Cell- derived liver cells from healthy and diseased donors: A valuable tool for modeling inflammation and fibrosis

Datum

22.09.2023

Zeit

11:00 - 12:00

Sprecher

Aina, Kehinde O.

Zugehörigkeit

Marie Curie Sklodowska Fellow, INSPIRE research group, Uniklinikum Jena

Sprache

en

Hauptthema

Biologie

Host

Meritxell Huch

Beschreibung

To improve the predictive power of in vitro models, vascular biology needs to be integrated into a functional, preferably facile, technology. The use of human induced pluripotent stem cells (hiPSC) in combination with micro-physiological systems, have emerged as tools to provide insight into the mechanisms of metabolic liver disorders, and liver fibrosis. A novel on-chip in vitro differentiation and tissue engineering procedure was used to generate a stem cell-based derived liver-on-chip model using a hiPSC panel of healthy and NASH donors in a 3D format. Liver fibrosis disease modeling was based on the stimulation of the model with transforming growth factor β. The fibrosis phenotype was validated based on endothelial cell dysfunction, stellate cell activation, and deposition of extracellular matrix. Isogenic LoC models would facilitate the study of patho-physiological processes involved in liver disease progression for personalized medicine approaches.

Letztmalig verändert: 23.09.2023, 07:35:33