Multi-module recording of neuronal activity and structures using graphene microelectrode arrays in the study of Niemann -Pick disease

Datum

05.12.2024

Zeit

13:00 - 15:00

Sprecher

Meng Lu

Zugehörigkeit

Institute of Advanced Clinical Medicine, Peking University

Serie

TUD nanoSeminar

Sprache

en

Hauptthema

Physik

Andere Themen

Physik

Host

Arezoo Dianat

Beschreibung

Simultaneously recording network activity and ultrastructural changes of the synapse can significantly advance our understanding of the structural basis of neuronal functions. The intricate changes in neuronal activity at millisecond-scale and the minute structural modifications at the synapse that are smaller than the diffraction limit present considerable hurdles for this undertaking. Here, we introduce a multi-module recording system based on graphene microelectrode arrays (G-MEAs), which facilitate high-resolution imaging at different scales and permit electrophysiological recordings with high temporal precision. In conjunction with G-MEAs, we also apply a straightforward machine learning algorithm to streamline the analysis of extensive data gathered from microelectrode array recordings. We illustrate that the integration of G-MEAs, machine learning-based spike analysis, and four-dimensional structured illumination microscopy (4D-SIM) provides a powerful tool for observing the effects of disease progression on hippocampal neurons. Furthermore, treating neurons with an inhibitor of intracellular cholesterol transport to mimic Niemann-Pick disease type C (NPC) results in a significant enlargement of synaptic boutons compared to untreated neurons, ultimately impairing neuronal signaling capabilities.

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Letztmalig verändert: 21.11.2024, 07:40:28