Cell heterogeneity and fate bistability drive tissue patterning during intestinal regeneration

Datum

11.02.2025

Zeit

10:00 - 11:00

Sprecher

Cornelia Schwayer

Zugehörigkeit

ETH Zürich | Dept. for Biosystems Science and Engineering (D-BSSE) & Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland

Sprache

en

Hauptthema

Biologie

Host

Meritxell Huch

Beschreibung

Tissue regeneration relies on the ability of cells to undergo de novopatterning. While tissue patterning has been viewed as the transition from initially identical un-patterned cells to an arrangement of different cell types, recent evidence suggests that initial heterogeneities between cells modulate tissue-scale pattern formation. Yet, how such heterogeneities arise and, thereafter, regulate cell type emergence in a population of cells is poorly understood. Using in vivo and in vitro mouseregenerative systems, we identify a critical tissue density that is required to induce heterogeneous inactivation of the mechanosensor YAP1. Experimental and biophysical approaches demonstrate that YAP1 cell-to-cell heterogeneity pre-patterns the first cell fate decision, via both chromatin remodelling and a supracellular feedback between FOXA1 and Delta-Notch signalling. This feedback motif induces cell fate bistability endowing memory to the system and the maintenance of patterns during homeostasis. These findings reveal a generalisable framework in which transient cell-to-cell heterogeneity, regulated by tissue-scale properties, serves as a critical control parameter for the emergence of cell fate and stable patterning during regeneration.

Letztmalig verändert: 11.02.2025, 07:35:30