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UID:DSC-3572
DTSTART;TZID=Europe/Berlin:20120803T110000
SEQUENCE:1343645074
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20120803T120000
URL:https://dresden-science-calendar.de/calendar/de/detail/3572
LOCATION:TUD CRTD\, Fetscherstraße 10501307 Dresden
SUMMARY:Gebhardt: Probing cellular processes with in vitro and in vivo sing
 le molecule experiments
CLASS:PUBLIC
DESCRIPTION:Speaker: Dr. Christof Gebhardt\nInstitute of Speaker: Harvard U
 niversity\, Department of Chemistry and Chemical Biology\nTopics:\nBiologi
 e\, Chemie\n Location:\n  Name: TUD CRTD (Auditorium left)\n  Street: Fets
 cherstraße 105\n  City: 01307 Dresden\n  Phone: +49 (0)351 458 82052\n  F
 ax: +49 (0)351 458 82059 \nDescription: Monitoring the stochastic actions 
 of a single biomolecule reveals details of its functioning hidden in ensem
 ble measurements and thus enables a comprehensive mechanistic understandin
 g of cellular processes. While single molecule approaches are well establi
 shed in vitro\, as demonstrated here with optical tweezers force spectrosc
 opy experiments on protein folding and the stepping mechanism of a molecul
 ar motor\, following individual biomolecules in vivo is challenging. In or
 der to monitor single protein molecules in their natural environment in a 
 mammalian nucleus\, we developed a novel fluorescence microscopy method ba
 sed on selective plane illumination\, Reflected Light Sheet Microscopy (RL
 SM). With the new microscope\, we quantitatively measured DNA residence ti
 mes of transcription factors and probed the complex formation between two 
 factors and DNA. In combination with super-resolution microscopy\, we mapp
 ed the positions of RNA polymerase throughout the nucleus.  
DTSTAMP:20260623T175517Z
CREATED:20120730T104434Z
LAST-MODIFIED:20120730T104434Z
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