Towards Large Scale Reconstruction of Neuro-Anatomy at Nanometer Resolution

Datum

25.02.2013

Zeit

14:00 - 15:00

Sprecher

Dipl.-Medieninf. Stephan Saalfeld

Sprache

en

Hauptthema

Informatik

Andere Themen

Informatik

Beschreibung

A classic method for high resolution neuronal circuit reconstruction is to image a volume of neuronal tissue as a series of ultra-thin sections, each as a mosaic of overlapping tiles. In order to reconstruct the volume, the individually prepared and imaged sections need to be re-assembled into a continous 3d space. Each section and tile may be deformed significantly. I describe a new registration method that simultaneously maximizes the overlap of corresponding regions and minimizes the deformation applied to each single image through a global elastic constraint. The method has been used to automatically restore large Electron Microscopy section series consisting of more than 33,000 individual images.

Letztmalig verändert: 25.02.2013, 08:38:36