Celigo®: Imaging Cytometer in Cancer Applications and basic research / Multiparametric SPR: Novel insights from an old technique and how it can impact membrane biophysics

Datum

11.02.2014

Zeit

10:00 - 11:00

Sprecher

Cenibra

Zugehörigkeit

Cenibra

Sprache

en

Hauptthema

Biologie

Andere Themen

Biologie

Host

MPI-CBG Technology Day 2014

Beschreibung

Celigo®: Imaging Cytometer in Cancer Applications and basic research The challenges faced daily by biologists in cell based assays are multifactorial and numerous, from disruptive techniques to time consuming manual cell based microscopy analysis and single point reads. We focus here on the Celigo Imaging Cytometer for cell based cancer applications and basic research bottlenecks from simple cell counts, confluence and viability to more complex cell death pathway analysis and 3D Tumorsphere analysis. These newer greater throughput imaging cytometric techniques promise to give a greater depth of relevant data to allow more rapid combinatorial knowledge in addition to allowing researchers to focus on less routine and time consuming manual analyses. Multiparametric SPR – novel insights from an old technique and how it can impact membrane biophysics The look at the angular shift in surface plasmon resonance has become a standard tool in biochemical binding analyses. However, the narrow window the traditional SPR-approach is using limits both the applicability to biomembrane related questions and the flexibility with regard to possible other coatings. With their multiparametric SPR-technology BioNavis give access to biophysical parameters of coated membranes and even coated cells. The look at the entire SPR-curve reveals phenomena such as total internal reflection and hence allows access to new experiments and new answers on hitherto difficult sample classes.

Letztmalig verändert: 12.02.2014, 08:41:35