FCS-based single molecule analysis of Fgf8 gradient formation in zebrafish

Datum

12.03.2014

Zeit

15:40 - 16:40

Sprecher

Mansi Gupta

Zugehörigkeit

Brand Lab, BIOTEC/CRTD, Dresden

Sprache

en

Hauptthema

Biologie

Andere Themen

Biologie

Host

BioDIP Spring Seminar

Beschreibung

Proteins and their interactions are of central importance in biology, e.g. in every signalling cascade. Fluorescence (Cross-)Correlation Spectroscopy (F(C)CS) offers the possibility to investigate proteins and their interactions live, both in vitro and in vivo. To achieve that, F(C)CS analyzes the movement of fluorescent particles into and out of a focal volume at a confocal microscope and can deduce several parameters from this analysis. (1) FCS measures the mobility and concentration of a fluorescently tagged molecule of interest and thereby provides information about protein concentrations and associations. (2) FCCS uses two, fluorescently tagged molecules and yields quantitative data describing the interaction of these molecules (dissociation constant). In the BioDIP seminar we will span the range from the investigation of protein complexes in solution to measurements of protein interaction in the membrane of the living embryo. If you are curious to learn how you can use F(C)CS for probing the dynamics/interactions of your protein of interest, join us for the seminar and the discussion afterwards.

Letztmalig verändert: 13.03.2014, 08:50:40