Proteomic characterization of high-grade serous ovarian carcinoma using SWATH-MS and iTRAQ DDA in comparison

Date

Nov 16, 2017

Time

2:30 PM - 3:30 PM

Speaker

Betty Friedrich-Grube

Affiliation

Institute of Molecular Systems Biology, ETH Zürich

Series

IMB - Seminar

Language

en

Main Topic

Biologie

Other Topics

Biologie, Medizin

Host

IMB

Description

A highly aggressive subtype of ovarian cancer called high-grade serous ovarian carcinoma (HGS-OC) accounts for 70% of the deaths from ovarian cancer and has an estimated 5-year survival rate of 31%. In this study we would like to characterize the proteomic landscape of >100 HGS-OC samples previously analyzed by The Cancer Genome Atlas (TCGA) as well as the Clinical Proteomic Tumor Analysis Consortium (CPTAC) (Zhang et al, 2016. Cell). The CPTAC approach consists of a complex and deep analysis usingiTRAQ-labeled, data-dependent acquisition, whereas SWATH-MS is a massively parallel targeted, label-free, data-independent mass spectrometry method. Comparing the results from those two tremendously different mass spectrometry techniques, SWATH-MS and iTRAQ-DDA, we can identify robust biological information and gain conclusions for the design of future studies using clinical data.

Last modified: Nov 1, 2017, 11:46:31 AM