Hit Identification & Confirmation Strategies in Arrayed CRISPR-Cas9 Screening
- Date
- Nov 17, 2017
- Time
- 11:00 AM - 12:00 PM
- Speaker
- Louise Baskin
- Affiliation
- Senior Scientific Product Manager, Dharmacon
- Language
- en
- Main Topic
- Biologie
- Other Topics
- Biologie
- Host
- Marc Bickle
- Description
- • Execution of a synthetic crRNA library screen using high content imaging identify potential hits important in the cell cycle • Statistical examination of six phenotypic parameters reveal robust, functional knockout for multiple reagents per gene • Follow-up experiments including confirmation of phenotype in an independent experiment, expression analysis, and editing efficiency will be discussed • Gene annotation and crRNA target position analysis give novel insights into system biology • Confirmation of hits with orthogonal reagents (siRNA) supports high-confidence hits • Strategies for further validation and hit stratification will be discussed
Last modified: Nov 18, 2017, 8:37:27 AM
Location
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Galleria)Pfotenhauerstraße10801307Dresden
- Phone
- +49 351 210-0
- Fax
- +49 351 210-2000
- MPI-CBG
- Homepage
- http://www.mpi-cbg.de
Organizer
Max Planck Institute of Molecular Cell Biology and GeneticsPfotenhauerstraße10801307Dresden
- Phone
- +49 351 210-0
- Fax
- +49 351 210-2000
- MPI-CBG
- Homepage
- http://www.mpi-cbg.de
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