BEGIN:VCALENDAR
VERSION:2.0
PRODID:www.dresden-science-calendar.de
METHOD:PUBLISH
CALSCALE:GREGORIAN
X-MICROSOFT-CALSCALE:GREGORIAN
X-WR-TIMEZONE:Europe/Berlin
BEGIN:VTIMEZONE
TZID:Europe/Berlin
X-LIC-LOCATION:Europe/Berlin
BEGIN:DAYLIGHT
TZNAME:CEST
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
DTSTART:19810329T030000
RRULE:FREQ=YEARLY;INTERVAL=1;BYMONTH=3;BYDAY=-1SU
END:DAYLIGHT
BEGIN:STANDARD
TZNAME:CET
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
DTSTART:19961027T030000
RRULE:FREQ=YEARLY;INTERVAL=1;BYMONTH=10;BYDAY=-1SU
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
UID:DSC-13752
DTSTART;TZID=Europe/Berlin:20171116T100000
SEQUENCE:1510818909
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20171116T110000
URL:https://dresden-science-calendar.de/calendar/en/detail/13752
LOCATION:IFW\, Helmholtzstraße 2001069 Dresden
SUMMARY:Xu: Sperm as a drug carrier: multi-sperm transport and propulsion f
 orce measurement
CLASS:PUBLIC
DESCRIPTION:Speaker: Haifeng Xu\nInstitute of Speaker: Institut für Integr
 ative Nanowissenschaften (IIN)\nTopics:\nMaterialien\, Physik\n Location:\
 n  Name: IFW (D2E.27\, IFW Dresden)\n  Street: Helmholtzstraße 20\n  City
 : 01069 Dresden\n  Phone: \n  Fax: \nDescription: The sperm\, as a combina
 tion of a naturally selected micromotor and cellular drug carrier\, shows 
 a great potential on drug delivery to treat diseases in the female reprodu
 ctive tract. Owing to its crystalline nucleus1 and compact membrane system
 \, the sperm can encapsulate high amount of drug and protect it from body 
 fluid dilution. After a precise guidance with a specific magnetic structur
 e\, the sperm can release drug and enhance the transfer of it into cancer 
 cells relying on its cell-fusion ability.2 In previous work\, we showed th
 e successful loading of BSA into sperms and fabrication of tetrapod-like s
 tructures\, as well as the sperm release when hitting PDMS walls and HeLa 
 cells by mechanical deformation of tetrapod arms.  Here\, we present the c
 ompletion and improvement of the study of sperm-tetrapod micromotor for th
 e delivery of DOX-HCl (doxorubicin hydrochloride\, anticancer drug). The s
 perm exhibited an impressive encapsulation capability of DOX-HCl\, carryin
 g an amount of ca. 15 pg of drug in one sperm. No significant difference w
 as found on sperm motility between DOX-HCl-loaded sperms and unloaded sper
 ms\, making them sufficiently active for subsequent transport. Besides\, D
 OX-HCl-loaded sperms showed a more efficient cell-killing effect on HeLa s
 pheroids compared to DOX-HCl solution with the same amount of drug. To dem
 onstrate the potential application of this system on targeted drug deliver
 y\, DOX-HCl-loaded sperms were coupled in tetrapods and guided to HeLa sph
 eroids. Fluorescence spreading indicated the drug distribution from sperm 
 to HeLa cells (Fig. 1a)\, leading to the cancer cell apoptosis. SEM images
  confirmed the drug effect on a cancer cell by a fused drug-loaded sperm. 
   Despite the experimental achievement in vitro\, there are still several 
 challenges to apply this in vivo\, such as real-time imaging\, biodegradab
 ility and effective drug dose. Thus\, we propose a multi-sperm transport s
 ystem (Fig. 1b) by using hyaluronic acid (HA) micro-beads as a sperm attra
 ction platform to improve the drug dose by capturing multiple sperms. Prel
 iminary results showed that HA micro-beads could efficiently attract multi
 ple sperms and be delivered to a target by using a big magnetic helical tr
 ansporter. After that\, sperms were able to escape due to the slow dissolu
 tion of HA. This development brought us a promising strategy to enhance th
 e effective drug dose.  In addition\, we suggest a force-measurement devic
 e (Fig. 1c) to evaluate the propulsion power of the sperm for a better und
 erstanding on its cumulus cells or tissue penetration ability.  We believe
  that this novel approach of using sperm-hybrid micromotors as drug carrie
 rs will represent a great breakthrough in the treatment of gynecological c
 ancers in the future.  References: 1. Johnson\, G. D.\; Lalancette\, C.\; 
 Linnemann\, A. K.\; Leduc\, F.\; Boissonneault\, G.\; Krawetz\, S. A.\, Th
 e sperm nucleus: chromatin\, RNA\, and the nuclear matrix. Reproduction 20
 11\, 141\, 21-36. 2. Mattioli\, M.\; Gloria\, A.\; Mauro\, A.\; Gioia\, L.
 \; Barboni\, B.\, Fusion as the result of sperm-somatic cell interaction. 
 Reproduction 2009\, 138\, 679-87.
DTSTAMP:20260626T084538Z
CREATED:20171111T080500Z
LAST-MODIFIED:20171116T075509Z
END:VEVENT
END:VCALENDAR