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UID:DSC-18264
DTSTART;TZID=Europe/Berlin:20211108T161500
SEQUENCE:1636380598
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20211108T174500
URL:https://dresden-science-calendar.de/calendar/en/detail/18264
LOCATION:B CUBE\, Tatzberg 4101307 Dresden
SUMMARY:Mitra: A single molecule approach to decipher assembly and entry  o
 f trains into C. elegans sensory cilia
CLASS:PUBLIC
DESCRIPTION:Speaker: Aniruddha Mitra\nInstitute of Speaker: Department of P
 hysics and Astronomy\, and LaserLaB Amsterdam\, Vrije Universiteit Amsterd
 am\, The Netherlands\nTopics:\nBiologie\n Location:\n  Name: B CUBE (Semin
 ar Rooms E73+ E74)\n  Street: Tatzberg 41\n  City: 01307 Dresden\n  Phone:
  +49 351 463 43000\n  Fax: +49 351 463 40322\nDescription: Sensory neurons
  of C. elegans have signalling hubs called primary cilia\, extending from 
 dendritic endings\, to sense and respond to the external environment. Cili
 a have a microtubule-based intraflagellar transport (IFT)\, which is essen
 tial for the growth and maintenance of the structure. The IFT proteins ass
 ociated with cilia\, have to be transported from the cell body to the cili
 a base\, moving across the dendrites (10s of microns)\, where they assembl
 e into IFT trains and cross a transition barrier to enter the cilia. By pe
 rforming single-molecule fluorescence microscopy in the chemosensory neuro
 ns of C. elegans\, we unravel that while motor proteins involved in IFT\, 
 kinesin-II\, OSM-3 and IFT dynein\, move diffusively from the cell soma to
  the cilia base\, other proteins like IFT train proteins (CHE-11 and OSM-6
 ) and ciliary transmembrane protein (OCR-2) are transported as packets in 
 a directional manner. Single-particle tracking reveals that these packets 
 show rich pausing dynamic\, as they approach the cilia base\, before docki
 ng at the cilia base. Further\, we find that the IFT train proteins and IF
 T dynein pause at the base for a prolonged time\, incorporating into IFT t
 rains during the assembly process\, before entering the cilia. In contrast
 \, kinesin-II and OSM-3 are picked up by pre-packaged IFT trains\, directl
 y at the cilia base in case of kinesin-II and throughout the proximal segm
 ent of the cilia in case of OSM-3. Thus\, using single-molecule live cell 
 imaging in C. elegans\, we illuminate how IFT proteins reach the cilia bas
 e\, assemble into IFT trains and enter the cilia.
DTSTAMP:20260524T132322Z
CREATED:20211108T140958Z
LAST-MODIFIED:20211108T140958Z
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