CMCB Life Science Seminar: Prof. Sonja Schmid, Wageningen University & Research, Labortatory of Biophysics, The Netherlands
- Sep 21, 2023
- 1:00 PM - 2:00 PM
- Main Topic
- Other Topics
Host: Michael Schlierf (B CUBE)
Title: “Dissecting the molecular driving forces of life with single-molecule resolution”
Abstract: Proteins are the molecular makers in our body. Researchers successfully identified a vast proteome, a dense web of metabolic interactions, and many thousands of static 3D structures. But the essential dynamic processes causing protein function are still challenging to detect – yet they are the key to the energetics controlling life at the nanoscale. We therefore focus on acquiring time-resolved information to reveal the nano-dynamics of biomolecular systems, such as cancer-assisting chaperone, kinase proteins, and CRISPR-associated proteins.
In this talk, I will present our latest results from optical and electrical single-molecule experiments. FRET allows us to watch single proteins at work in real time [1,2,3], and with our DyeCycling approach , we can now overcome its infamous photobleaching limitation and detect previously hidden kinetic phenomena. In addition, we recently presented the NEOtrap 2.0, a label-free technique to monitor the time evolution of single unmodified proteins electrically, using nanopores [5,6]. Lastly, I will share how we revealed the hidden power of type-III CRISPR-Cas – one-by-one. Our overarching mission is to push beyond current detection limits, to learn how biomolecular function arises at the nanoscale.
Last modified: Sep 21, 2023, 7:35:58 AM
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