Mitotic centrosomes are assembled upon inter-dependent solid- and liquid-like scaffolds

Date

Sep 22, 2023

Time

11:00 AM - 12:00 PM

Speaker

Isaac Siu-Shing Wong

Affiliation

Sir William Dunn School of Pathology, University of Oxford

Language

en

Main Topic

Biologie

Host

Tony Hyman

Description

Mitotic centrosomes are formed when centrioles recruit pericentriolar material (PCM) around themselves. The PCM comprises several hundred proteins, and its physical nature is hotly debated. In flies, Spd-2/CEP192 recruits Polo/PLK-1 and Cnn/CDK5RAP2 (fly/human nomenclature) to the centriole, and these form a solid-like scaffold that recruits PCM clients. Here we show that Spd-2 also recruits a liquid-like scaffold to centrioles that depends on Aurora A and TACC and is analogous to the liquid-like spindle domain (LISD) in mammalian acentrosomal meiotic spindles. The Polo/Cnn and Aurora A/TACC scaffolds can assemble and recruit PCM clients independently of one another, but centrosome function is severely perturbed in the absence of either scaffold. Thus, mitotic centrosomes in flies assemble upon co-existing solid- and liquid-like scaffolds organised by Spd-2/CEP192 and either Polo/PLK1 or Aurora A, respectively.

Last modified: Sep 23, 2023, 7:35:33 AM