Electrochemical Immunoassay-based Sensors Towards Point-Of-Care Diagnostics: Recent Progress and Challenges in Multiple Biomarkers Detection

Date

Nov 7, 2024

Time

1:00 PM - 3:00 PM

Speaker

Zuzana Bílková and Lucie Korecká

Affiliation

Department of Biological and Biochemical Sciences, Faculty of Chemical-Technology, Czech Republic

Series

TUD nanoSeminar

Language

en

Main Topic

Physik

Other Topics

Physik

Host

Arezoo Dianat

Description

Specific biomarkers detected in body fluids and tissues are crucial elements for disease diagnosis, prediction of its progression, and evaluation of personalised treatment effectiveness. Although the detection of a single specific biomarker is sufficient, the simultaneous detection of multiple disease-associated biomarkers (cancer biomarkers, inflammatory proteins, etc.) significantly improves the diagnostic power of biomarkers in terms of specificity and sensitivity from the Receiver operating characteristic curve (ROC).
 Electrochemical biosensors meet the criteria of Point-of-Care (POCT) devices and represent the alternative to commonly used instrumentally and time-consuming laborious diagnostic methods (ELISA, immunohistochemistry, PCR). Among electrochemical biosensors, immunosensors, based on the principle of highly specific affinity interactions between antigens and two corresponding antibodies identical to the ELISA method, are of interest in clinical diagnosis. Increasingly, the common enzyme-based immunosensors are being replaced by nanomaterials-based sensors to overcome the shortcomings of enzymes. From electroactive nanoparticles, gold nanoparticles (AuNPs), silver nanoparticles (AgNPs), quantum dots (QDs), carbon nanotubes (CNTs), graphene, and carbon quantum dots (CQDs) are the most commonly used. Moreover, these nanomaterials enable the simultaneous detection of multiple biomarkers since they provide separate, non-interfering and mutually unaffected electrochemical signals.
 The talk will be focused on the development of electrochemical magneto-immunoassay-based sensors for simultaneous stripping voltammetric detection of ovarian cancer biomarkers, namely proteins HE4, CA125, AFP, and pro-inflammatory biomarkers PTX3 and CALR, as potential predictive markers of preterm labour.

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Last modified: Nov 7, 2024, 9:14:55 AM