Microfluidic Manipulation and Measurement of Cell Mechanics for Medicine

Date

Oct 7, 2014

Time

11:00 AM - 12:00 PM

Speaker

Prof. Dino Di Carlo University of California, Los Angeles Microfluidic Biotechnology Laboratory California NanoSystems Institute

Language

en

Main Topic

Biologie

Other Topics

Biologie, Medizin

Host

Jochen Guck

Description

Please contact the host ( ) if you would like to talk to the speaker. Everybody is welcome! Abstract: Cell deformability (i.e., the ability to change shape under an applied force) is a promising physical marker indicative of underlying structural changes associated with various disease processes and changes in cell state. We are combining precision microfluidic control of cells with automated high-speed image analysis for high-throughput cell classification based on intrinsic biomechanical properties. I will first discuss general strategies we are developing to passively manipulate particles and fluids using simple geometric modifications within microchannels. Our approaches make use of fluid inertia, generally neglected in microfluidic systems, to create well-defined directional forces and fluid deformations that can be combined in a sequential and hierarchical manner to program complex particle and fluid motions. Low complexity modular components to manipulate cells, particles, and fluid streams in which inertial fluid physics is abstracted from the designer has the capability transform biological, chemical, and materials automation in a similar fashion to how modular control of electrons and abstraction of semiconductor physics transformed computation. We apply these fundamental techniques to position cells for high-speed fluid-based deformation and optical analysis. The “deformability cytometer” instrument shows promise in identifying cancer cells, activated white blood cells, and stem cells in mixed populations – without labels - for a variety of clinical and regenerative medicine applications. Recent Publications: Tse HTK, Gossett DR, Moon YS, Masaeli M, Sohsman M, Ying Y, Mislick K, Adams RP, Rao J, Di Carlo D. Quantitative Diagnosis of Malignant Pleural Effusions by Single-Cell Mechanophenotyping. Science Translational Medicine (2013). doi: 10.1126/scitranslmed.300655 Sollier E, Go DE, Che J, Daniel Gossett, Sean O'Byrne, Weaver WM, Kummer N, Rettig M, Goldman J, Nickols N, McCloskey S, Kulkarni R and Di Carlo D. Size-Selective Collection of Circulating Tumor Cells using Vortex Technology. Lab on a Chip (2013). doi: 10.1039/C3LC50689D Amini H, Sollier E, Masaeli M, Xie Y, Ganapathysubramanian B, Stone HA, and Di Carlo D. Engineering fluid flow using sequenced microstructures. Nature Communications (2013). doi:10.1038/ncomms2841 Tseng, P, Judy JW, Di Carlo D. Magnetic nanoparticle-mediated massively parallel mechanical modulation of single-cell behavior. Nature Methods (2012). doi:10.1038/nmeth.2210 Gossett DR, Tse HTK, Lee SA, Ying Y, Lindgren AG, Yang OO, Rao J, Clark AT, Di Carlo D. Hydrodynamic stretching of single cells for large population mechanical phenotyping. PNAS (2012) doi: 10.1073/pnas.1200107109.

Links

• to announcement of the organizer

Last modified: Oct 7, 2014, 9:37:06 AM