The Edge of the Nucleus

Datum

14.03.2014

Zeit

10:30 - 11:30

Sprecher

Donald and Ada Olins, University of New England, USA

Sprache

en

Hauptthema

Willkommen

Andere Themen

Willkommen

Host

Jochen Guck

Beschreibung

Our studies have focused upon the chromatin adjacent to the nuclear envelope (NE). Most experiments have been conducted with the human leukemia HL-60 cell line, which can be differentiated in vitro. The granulocyte forms exhibit ELCS (envelope-limited chromatin sheets) connecting the nuclear lobes. Evidence is presented that ~10 nm chromatin fibers adjacent to the NE are organized into a crisscross pattern that resembles ~30 nm fibers. Epichromatin, the surface chromatin, in all the HL-60 cell states has unique immunological properties that have been exploited for ChIP-Seq analyses. Epichromatin appears to be highly enriched in retrotransposon Alu, and exhibits spatial patterns that are chromosome specific and independent of the state of cell differentiation.

Links

• to announcement of the organizer

Letztmalig verändert: 14.03.2014, 08:42:23