Preclinical validation of an hESC-retinal epithelial cell sheets in rodents and primates

Date

Apr 12, 2018

Time

4:00 PM - 5:00 PM

Speaker

Prof. Christelle Monville

Affiliation

I-Stem, INSERM/UEVE UMR861

Language

en

Main Topic

Biologie

Other Topics

Biologie, Medizin

Host

Dr. Madalena Carido Pereira

Description

Abstract: Loss of retinal pigmented epithelial (RPE) cells is responsible for severe vision impairments in retinal degenerative disorders, such as retinitis pigmentosa. Stem cell-derived RPE cells have shown promise as a cell therapy for treating retinal degeneration in preclinical studies, but long-term positive outcomes require efficient engraftment and survival of the transplanted cells. Taking a step in this direction, we have developed a tissue engineered product comprising human embryonic stem cell (hESC)-derived RPE cells that were grown as sheets on a biological scaffold. Transplantation of these hESC-RPE cell sheets into a rat model of retinal degeneration resulted in greater cell engraftment and survival and improved visual acuity compared to hESC-RPE cells transplanted as cell suspensions. These tissue engineered cell sheets may improve the efficacy of cell therapy for treating retinal degenerative diseases 5 most important publications: 1. Ben M'Barek K, Habeler W, Plancheron A, Jarraya M, Regent F, Terray A, Yang Y, Chatrousse L, Domingues S, Masson Y, Sahel JA, Peschanski M, Goureau O, Monville C. Clinically compatible human ES-derived RPE cells grafted as an epithelium potentiates vision rescue in dystrophic rodent. Sci Transl Med. 2017 Dec 20;9(421). 2. Ben M'Barek K, Regent F, Monville C. Use of human pluripotent stem cells to study and treat retinopathies. World J Stem Cells. 2015 Apr 26;7(3):596-604. 3. Reichman S, Terray A, Slembrouck A, Nanteau C, Orieux G, Habeler W, Nandrot E, Sahel JA, Monville C, Goureau O. From confluent human iPS cells to self-forming neural retina and RPE cells. PNAS. (2014) 111(23):8518-23. 4. Lustremant C, Habeler W, Plancheron A, Goureau O, Grenot L, de la Grange P,Audo I, Nandrot EF, Monville C. Human induced pluripotent stem cells as a tool to model a form of Leber congenital amaurosis. Cell Reprogram. (2013) 15(3):233-246 5. Habeler W, Ghaleh B , Barthelemy I, Plancheron A, Bizé A, Carlos-Sampedrano C, Chetboul V, Berdeaux A, Pucéat M, Peschanski M, Blot S, Monville C. Direct myocardial implantation of human embryonic stem cells in a dog model of Duchenne cardiomyopathy reveals poor cell survival in dystrophic tissue. JSRM (2011) 7(2): 80-86.

Links

• to announcement of the organizer

Last modified: Apr 12, 2018, 9:40:30 AM