Single-cell delineation of lineage and genetic identity in the mouse brain

Date

Mar 30, 2023

Time

11:00 AM - 12:00 PM

Speaker

Christian Mayer

Affiliation

MPI of Neurobiology & MPI for Biological Intelligence Martinsried, Germany

Series

MPI-CBG Thursday Seminar

Language

en

Main Topic

Biologie

Host

Marino Zerial

Description

During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of post-mitotic neurons and glia. The talk will discuss the link between developmental lineage and cell type diversity, which is still an open question in the field. We used massively parallel tagging of progenitors to track clonal relationships and transcriptomic signatures during mouse forebrain development. We quantified clonal divergence and convergence in all major cell classes of the mouse forebrain and found several types of GABAergic neurons that share a common lineage. Divergence of GABAergic clones occurred during embryogenesis at cell cycle exit, suggesting that differentiation into subtypes is initiated as a lineage-dependent process at the progenitor level. The talk will also discuss a mechanism by which selective enhancer activation is critical for establishing GABAergic projection neuron identity and potential pathological mechanisms.

Last modified: Mar 31, 2023, 7:39:49 AM