Observing cytoplasmic interactions of centrosome proteins using FCS

Date

Mar 12, 2014

Time

4:20 PM - 5:20 PM

Speaker

Oliver Wüseke

Affiliation

MPI-CBG, Hyman lab, Dresden, Germany

Language

en

Main Topic

Biologie

Other Topics

Biologie

Host

BioDIP Spring Seminar

Description

Proteins and their interactions are of central importance in biology, e.g. in every signalling cascade. Fluorescence (Cross-)Correlation Spectroscopy (F(C)CS) offers the possibility to investigate proteins and their interactions live, both in vitro and in vivo. To achieve that, F(C)CS analyzes the movement of fluorescent particles into and out of a focal volume at a confocal microscope and can deduce several parameters from this analysis. (1) FCS measures the mobility and concentration of a fluorescently tagged molecule of interest and thereby provides information about protein concentrations and associations. (2) FCCS uses two, fluorescently tagged molecules and yields quantitative data describing the interaction of these molecules (dissociation constant). In the BioDIP seminar we will span the range from the investigation of protein complexes in solution to measurements of protein interaction in the membrane of the living embryo. If you are curious to learn how you can use F(C)CS for probing the dynamics/interactions of your protein of interest, join us for the seminar and the discussion afterwards.

Last modified: Mar 13, 2014, 8:50:40 AM